Abstracts 2017

ABSTRACT: HOW CRISPR-BASED GENETIC TOOLS FACILITATE METABOLIC ENGINEERING OF YEAST

We would like to present to you the abstract of our keynote speaker Irina Borodina who is Senior Scientist and Group Leader at the Novo Nordisk Foundation Center for Biosustainability. 

Abstract

The majority of organic chemicals are derived from fossil sources. With the oil and gas resources becoming limiting, biotechnology offers a sustainable alternative for production of chemicals from renewable feedstocks. Yeast is an attractive cell factory for sustainable production of chemicals, due to its safe use status, tolerance of low pH and inhibitors, and amenability to large-scale fermentations.  The recent developments in CRISPR-based technology made genetic engineering of yeast cells cheaper and faster.

I will present how CRISPR-based genetic tools facilitate metabolic engineering of yeast Saccharomyces ceresivisiae1,2. Using these tools, we engineered yeast cell factories for production of chemicals from renewable feedstocks. Examples include non-native 3-hydroxypropionic acid3, a potential platform chemical for acrylics (diapers, acrylic paints, acrylic polymers, etc.) and aromatic secondary metabolites with applications as nutraceuticals and cosmetic ingredients (resveratrol4p-coumaric acid5, rosmarinic acid, flavonoids).

References

Stovicek et al (2015) “CRISPR-Cas system enables fast and simple genome editing of industrial Saccharomyces cerevisiae strains”. Metab Eng Commun, 2:13-22. DOI: 10.1016/j.meteno.2015.03.001.

Jessop-Fabre et al (2016) “EasyClone-MarkerFree: A vector toolkit for marker-less integration of genes into Saccharomyces cerevisiae“. Biotechnology J, 11(8):1110-1117. DOI: 10.1002/biot.201600147.

Borodina et al (2015) “Establishing a synthetic pathway for high-level production of 3-hydroxypropionic acid in Saccharomyces cerevisiae via β-alanine”. Metab Eng, 27:57-64.DOI: 10.1016/j.ymben.2014.10.003.

Li et al (2015) “De novo production of resveratrol from glucose or ethanol by engineeredSaccharomyces cerevisiae“. Metab Eng, 32:1-11. DOI: 10.1016/j.ymben.2015.08.007.

Rodriguez et al (2015) “Establishment of a yeast platform strain for production of p-coumaric acid through metabolic engineering of aromatic amino acid biosynthesis”. Metab Eng, 31:181-188. DOI: 10.1016/j.ymben.2015.08.003.